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Your Position: Home > Protein > Fc gamma RIV / CD16-2 > FC4-M52H3

Mouse Fc gamma RIV / CD16-2 Protein, His Tag (MALS verified)

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  • Synonym
    Fc gamma RIV,CD16-2,Fcgr4,FCGR4,mouse igg,Fc receptor
  • Source
    Mouse CD16-2, His Tag (FC4-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).
    Predicted N-terminus: Gly 21
  • Molecular Characterization
    Online(Gly 21 - Gln 203) A0A0B4J1G0-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.8 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) SDS-PAGE gel

Mouse CD16-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Mouse CD16-2, His Tag (Cat. No. ) MALS images

The purity of Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA-Series S sensor chip can bind OKT3 with an affinity constant of 154 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind OKT3 with an affinity constant of 140 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind Herceptin with an affinity constant of 122 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • Background
    FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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