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Human EGF R Protein, Fc Tag  pdf  pdf  pdf


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EGR-H5252-100ug
    $215.00
EGR-H5252-1mg (500ug x 2)
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Synonym

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

Source

Human EGF R, Fc Tag (EGR-H5252) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # NP_005219).

Predicted N-terminus: Leu 25

Molecular Characterization

EGF R(Leu 25 - Ser 645)NP_005219
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 95 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human EGF R, Fc Tag (Cat. No. EGR-H5252) SDS-PAGE gel

Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human EGF R, Fc Tag (Cat. No. EGR-H5252) ELISA bioactivity

(1) Immobilized Human EGF, Tag Free (Cat. No. EGF-H4114) at 10μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-3.2 μg/mL (QC tested).

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(2) Measured by its ability to bind Human EGF, Tag Free (Cat. No. EGF-H4114) in the SPR assay (Biacore 2000) with an estimated KD < 1nM. Validated CetuxiMab binding in a functional ELISA (Routinely tested).

 

Citations

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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