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Human EGF R Protein  pdf  pdf  pdf


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EGR-H5222-25ug
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EGR-H5222-50ug
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EGR-H5222-1mg (500ug x 2)
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Synonym

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

Source

Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).

Predicted N-terminus: Leu 25

Molecular Characterization

EGF R(Leu 25 - Ser 645)P00533-1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human EGF R, His Tag (Cat. No. EGR-H5222) SDS-PAGE gel

Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Human EGF R, His Tag (Cat. No. EGR-H5222) ELISA bioactivity

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with a linear range of 0.8-6 ng/mL (QC tested).

Request for protocol

Human EGF R, His Tag (Cat. No. EGR-H5222) ELISA bioactivity

Immobilized Erbitux to 200 RU on CM5 Chip via anti-human Fc IgG, can bind Human EGF R Protein (Cat. No. EGR-H5222) with the concentration of EGF R ranging from 0.125 nM to 4 nM. The affinity constant is 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Request for protocol

 

Citations

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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