Mouse CD3 epsilon Protein, Fc Tag, Asp 23 - Asp 108 (Accession # P22646) was produced in human 293 cells (HEK293) at ACROBiosystems.
Mouse CD3 epsilon, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 36 kDa. The predicted N-terminus is Asp 23. The reducing (R) protein migrates as 45 kDa in SDS-PAGE.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Trehalose is added as protectant before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
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