Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Bio-Markers & CD Antigens

Mouse CD3 epsilon Protein, Fc Tag  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
CDE-M5256-100ug
    $350.00
CDE-M5256-1mg
    $1980.00
In-Stock
Place Purchase Orders


Synonym

FLJ18683,T3E,TCRE,CD3E,CD3-epsilon

Source

Mouse CD3 epsilon, Fc Tag (CDE-M5256) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (Accession # P22646).

Predicted N-terminus: Asp 23

Molecular Characterization

CD3E(Asp 23 - Asp 108)P22646
Fc(Glu 99 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 36 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse CD3 epsilon, Fc Tag (Cat. No. CDE-M5256) SDS-PAGE gel

Mouse CD3 epsilon, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Related Products

Questions & Comments