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Human CD3 epsilon & CD3 gamma Heterodimer Protein  pdf  pdf  pdf


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Synonym

CD3 epsilon & CD3 gamma,CD3E & CD3G

Source

Human CD3 epsilon & CD3 gamma (CDG-H52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).

Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

Molecular Characterization

CD3E (Asp 23 - Asp 126)NP_000724.1
Fc(Pro 100 - Lys 330)P01857
Poly-his
CD3G (GIn 23 - Ser 116)AAI13831
Fc(Pro 100 - Lys 330)P01857
Flag

The protein contains two subunits. Subunit CD3E is fused with Fc tag and his tag at the C-terminus and subunit CD3D is fused with Fc tag and flag tag at the C-terminus.

The protein has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CD3 epsilon & CD3 gamma (Cat. No. CDG-H52W9) SDS-PAGE gel

Human CD3 epsilon & CD3 gamma on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Human CD3 epsilon & CD3 gamma (Cat. No. CDG-H52W9) ELISA bioactivity

Immobilized OKT3 MAb at 10μg/mL (100 μL/well) can bind Human CD3E & CD3G (Cat. No. CDG-H52W9) with a linear range of 0.3-5 μg/mL (QC tested).

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Human CD3 epsilon & CD3 gamma (Cat. No. CDG-H52W9) ELISA bioactivity

Immobilized SP34-2 at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3G, His Tag, Fc Tag, Flag Tag (Cat. No. CDG-H52W9) with a linear range of 0.6-10 ng/mL (Routinely tested).

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Background

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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