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Human Dkk-1 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

DKK1,SK

Source

Human Dkk-1 Protein, Fc Tag (Human Dkk-1, Fc Tag) Thr 32 - His 266 (Accession # NP_036374.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Dkk-1, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 52.4 kDa. The predicted N-terminus is Thr 32. The reducing (R) protein migrates as 70-90 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human Dkk-1, Fc Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Human Dkk-1 Protein, Fc Tag
The purity of Human Dkk-1, Fc Tag was determined by SDS-PAGE under reducing (R) condition and staining overnight with Coomassie Blue.
 
 

Background

Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

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References