Browse Products By Molecule:

Products --> Heavy Labeled Proteins

Human PCSK9 Protein, C13 and N15-labeled  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
PC9-H9223-20ug
    $1450.00
10 Weeks
Place Purchase Orders


Synonym

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

Source

Recombinant Human PCSK9 MS Standard Protein, C13 and N15-labeled (Human PCSK9, Heavy Labeled) Gln 31 - Gln 692 (Accession # AAI166619.1) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation efficiency at ACROBiosystems.

Molecular Characterization

C13 and N15-labeled, Human PCSK9 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 71.9 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 62 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.

Human PCSK9, Heavy Labeled is labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine

Endotoxin

Less than 1.0 EU per μg of the C13 and N15-labeled, Human PCSK9 by the LAL method.

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References