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Anti-PD-L1 Neutralizing Antibody  pdf  pdf  pdf


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PDL-NA002-100ug
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PDL-NA002-1mg
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Basic Information

Species Reactivity Human
Host Cells HEK293 cells
MW 50 and 25 kDa for the heavy and light chains, respectively (150 kDa)
Isotype Human IgG1, Kappa LC
Clonality Monoclonal

Description

Neutralizing recombinant human antibody recognizes the extracellular domain of human PD-L1 and blocks the binding of PD-L1 to PD-1. This antibody has not been tested for cross-reactivity with other species.

Specific Activity

IC50 = 3.75 nM using the PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Cat. # EP-101).

Purification

Protein A affinity chromatography from HEK293 supernatants.

Endotoxin

Less than 0.05 EU per μg by the LAL method.

Format

Lyophilized Powder

Application

Useful as a control inhibitor of PD-L1 binding using the PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Cat. # EP-101)

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
4 ℃ for 1 year in lyophilized state.
-70 ℃ for 3 months under sterile conditions after reconstitution.

 

Quality Assurance:

Inhibition of PD-1-PD-L1 binding by Anti-PD-L1 (CD274) Neutralizing Antibody (Catalog # PDL-NA002) measured using the PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair (Catalog # EP-101)

Anti-PD-L1 (CD274) neutralizing antibody (Cat. # PDL-NA002) was diluted from 20 ug/ml to 0.027 ug/ml and loaded onto the plate coated by human PD-L1 protein in the presence of biotinylated human PD-1 protein. Background was subtracted from data points prior to log transformation and curve fitting.

 
 

Background

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1). Immune checkpoint pathway is a focal point of today’s cancer research. PD-L1 is one of the best characterized checkpoint proteins. The binding between PD-L1 and its receptor PD1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, the pharmaceutical inhibition of PD-L1 or its receptor has been considered a promising strategy by many oncologists. The anti-PD-L1 (CD274) neutralizing antibody developed by ACRObiosystems specifically recognizes the extracellular domain of human PD-L1 and blocks the binding of PD-L1 to PD-1, and is very useful as positive control in inhibition assays.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.