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This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 25.5 kDa. The protein migrates as 36-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) was greater than 90% as determined by SEC-HPLC.
Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab biosimilar (Cat. No. CD0-M36) with an affinity constant of 0.367μM as determined in a SPR assay (Biacore 8K) (QC tested).
Immobilized Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) on SA Chip can bind Rituximab with an affinity constant of 0.3 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human CD16a (V176), Avitag,His Tag (SPR & BLI verified) (Cat. No. CDA-H82E9) on SA Biosensor, can bind Rituximab with an affinity constant of 0.316 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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