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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.3 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse B7-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) is more than 90% and the molecular weight of this protein is around 170-190 kDa verified by SEC-MALS.
Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 2 μg/mL (100 μL/well) can bind Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) with a linear range of 0.05-0.8 ng/mL (QC tested).
Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 5 μg/mL (100 μL/well) can bind Mouse PD-L1, mouse IgG2a Fc tag, low endotoxin (Cat. No. PD1-M52A2) with a linear range of 0.16-2.5 μg/mL (Routinely tested).
Immobilized Biotinylated Mouse CD28, His,Avitag (Cat. No. CD8-M82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) with a linear range of 0.6-10 ng/mL (Routinely tested).
Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) captured on CM5 chip via anti-human IgG Fc antibody can bind Mouse CD28, His Tag (Cat. No. CD8-M52H6) with an affinity constant of 49.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) on Protein A Biosensor, can bind Mouse CD28, His Tag (Cat. No. CD8-M52H6) with an affinity constant of 4.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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