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Human Angiopoietin-2 / ANGPT2 (275-496) Protein  pdf  pdf  pdf


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Synonym

ANGPT2,AGPT2,ANG2,Angiopoietin-2

Source

Human Angiopoietin-2 / ANGPT2 (275-496) Protein (Human Angiopoietin-2 (275-496), His Tag) Lys 275 - Phe 496 (Accession # AAI26201.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Angiopoietin-2 (275-496), His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 26.3 kDa. The predicted N-terminus is His. DTT-reduced Protein migrates as 30-33 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human Angiopoietin-2 (275-496), His Tag by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human Angiopoietin-2 / ANGPT2 (275-496) Protein
The purity of Human Angiopoietin-2 (275-496), His Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Angiopoietin-2 is also known as ANGPT2, AGPT2, ANG2, and is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. Both Ang2 and the related Angiopoietin1 (Ang1) are ligands for the receptor tyrosine kinase Tie 2. Ang2 functions as a proangiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang2 is required for postnatal vascular remodeling, and it cooperates with Ang1 during lymphatic vessel development. It mediates the upregulation of ICAM1 and VCAM1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang2 competitively inhibit Ang1-induced endothelial cell responses mediated by Tie2, and reduces vascular integrity. But the role of Ang2 is controversial since the opposite outcomes has been reported in other studies. Over-expression of Ang2 disrupts the vascular remodeling, induce endothelial cell apoptosis, and may play an important regulating role in tumor angiogenesis. Ang2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells.

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References

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