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Human PD-L1 / B7-H1 Protein, mouse IgG1 Fc Tag, low endotoxin (HPLC-verified)  pdf  pdf  pdf


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PD1-H52A3-100ug
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Synonym

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

Source

Human PD-L1, mouse IgG1 Fc Tag (PD1-H52A3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19

Molecular Characterization

PD-L1(Phe 19 - Arg 238)NP_054862.1
mFc(Val 98 - Lys 324)AAK53870.1

This protein carries a mouse IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.6 kDa. As a result of glycosylation, The protein migrates as 65-85 kDa under reducing (R) condition and 130-170 kDa under non-reducing (NR) condition on SDS-PAGE gel.

Endotoxin

Less than 0.1 EU per μg by the LAL method.

 

Purity

>92% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human PD-L1, mouse IgG1 Fc Tag (Catalog # PD1-H52A3) SDS-PAGE gel

Human PD-L1, mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

SEC-HPLC

Human PD-L1, mouse IgG1 Fc Tag (Catalog # PD1-H52A3) HPLC images

The purity of Human PD-L1 Protein, mouse IgG1 Fc Tag, low endotoxin (Cat.# PD1-H52A3) was more than 90% as determined by SEC-HPLC.

 

Bioactivity

Human PD-L1, mouse IgG1 Fc Tag (Catalog # PD1-H52A3) ELISA bioactivity

Immobilized Human PD-L1, mouse IgG1 Fc Tag (Catalog # PD1-H52A3) at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-1, His Tag & Fc tag (Catalog # PD1-H82F2) with a linear range of 0.05-0.2 μg/mL.

Request for protocol

Human PD-L1, mouse IgG1 Fc Tag (Catalog # PD1-H52A3) ELISA bioactivity

Immobilized Human PD-1, Tag Free, Full Length (Catalog # PD1-HC214) at 0.2 μg/mL ( 100 μl/well ) can bind Human PD-L1, mouse IgG1 Fc Tag (Catalog # PD1-H52A3) with a linear range of 2-80 ng/mL.

Request for protocol

 

Background

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

References

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