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Rhesus macaque PCSK9 Protein  pdf  pdf  pdf


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PC9-C52H2-50ug
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PC9-C52H2-1mg (500ug x 2)
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Synonym

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

Source

Rhesus macaque PCSK9, His Tag (PC9-C52H2) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # A8T666).

Predicted N-terminus: Gln 31

Molecular Characterization

PCSK9(Gln 31 - Gln 692)A8T666
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 73.2 kDa. The protein migrates as 18 kDa and 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) SDS-PAGE gel

Rhesus macaque PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 10 μg/mL (100 μl/well) can bind Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2 ) with a linear range of 2-15 ng/mL (QC tested).

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Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.