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Human IL-33 Protein, Tag Free  pdf  pdf  pdf


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IL3-H4112-20ug
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IL3-H4112-50ug
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Synonym

IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

Source

Human IL-33, Tag Free (IL3-H4112) is expressed from E.coli cells. It contains AA Ser 112 - Thr 270 (Accession # AAH47085).

Predicted N-terminus: Met

Molecular Characterization

IL-33(Ser 112 - Thr 270)AAH47085

This protein carries no "tag".

The protein has a calculated MW of 18.2 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IL-33, Tag Free (Cat. No. IL3-H4112) SDS-PAGE gel

Human IL-33, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Bioactivity

Human IL-33, Tag Free (Cat. No. IL3-H4112) ELISA bioactivity

Immobilized Human IL-33, Tag Free (Cat. No. IL3-H4112) at 5μg/mL (100 μL/well) can bind Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) with a linear range of 0.97-7.8 ng/mL (QC tested).

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Citations

Background

Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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