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Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling)  pdf  pdf  pdf


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Synonym

IL6,HGF,Interleukin-6,BSF2,HSF,IFNB2

Source

MABSol® Biotinylated Human IL-6 (IL6-H8218) is expressed from human HEK293 cells. It contains AA Val 30 - Met 212 (Accession # NP_000591). It is the biotinylated form of ActiveMax® Human IL-6 (Cat. No. IL6-H4218).

Predicted N-terminus: Val 30

Molecular Characterization

IL-6(Val 30 - Met 212)NP_000591

This protein carries no "tag".

The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio

The biotin to protein ratio is 4-5.5 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human IL-6 (Cat. No. IL6-H8218) SDS-PAGE gel

Biotinylated Human IL-6 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Biotinylated Human IL-6 (Cat. No. IL6-H8218) ELISA bioactivity

Immobilized Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) at 5 μg/mL (100 µL/well),can bind Biotinylated Human IL-6 (Cat. No. IL6-H8218) with a linear range of 4-250 ng/mL (QC tested).

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Background

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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