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Biotinylated Human Her2 / ErbB2, ultra sensitivity (primary amine labeling, long spacer)  pdf  pdf  pdf


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HE2-H822R-25ug
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Synonym

ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1

Source

MABSol® Biotinylated Human Her2 / ErbB2, Superior Sensitivity (HE2-H822R) is expressed from human HEK293 cells. It contains AA Thr 23 - Thr 652 (Accession # AAA75493). It is the biotinylated form of Human Her2 / ErbB2 Protein (Cat. No. HE2-H5225).

Predicted N-terminus: Thr 23

Molecular Characterization

Her2(Thr 23 - Thr 652)AAA75493
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.

Biotin:Protein Ratio

The biotin to protein ratio is 3.5-5.5 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human Her2, Superior Sensitivity (Cat. No. HE2-H822R) SDS-PAGE gel

Biotinylated Human Her2, Superior Sensitivity on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Biotinylated Human Her2, Superior Sensitivity (Cat. No. HE2-H822R) ELISA bioactivity

Immobilized Herceptin at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag (Cat. No. HE2-H822R) with a linear range of 0.8-25 ng/mL (QC tested).

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Citations

 

Background

Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2/neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.