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Cynomolgus PD-L1 / B7-H1 Protein  pdf  pdf  pdf


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Synonym

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

Source

Recombinant Cynomolgus PDL1 /CD274 Protein (Cynomolgus PDL1 /CD274) Phe 19 - Arg 238 was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

PD-L1 (Phe 19 - Arg 238)G7PSE7

polyhistidine

Cynomolgus PD-L1, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.1 kDa. The predicted N-terminus is Phe 19. The reducing (R) protein migrates as 35-40 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Cynomolgus PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Cynomolgus PD-L1, His Tag (Catalog # PD1-C52H4) at 10 μg/mL (100 μl/well) can bind Cynomolgus PD-1, Fc Tag (Catalog # PD1-C5254) with a linear range of 0.6-10 μg/mL.

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Background

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

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References