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Rhesus macaque CD19 (20-292) Protein, Fc Tag  pdf  pdf  pdf


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Synonym

CD19,B4,CVID3,MGC12802

Source

Recombinant Rhesus Macaque CD19 (20-292) Protein, C-Fc Tag (Rhesus Macaque CD19 (20-292) Fc Chimera) Pro 20 - Lys 292 (Accession # F7F486) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Rhesus Macaque CD19 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 56.7kDa. The predicted N-terminus is Pro 20. The reducing (R) protein migrates as 66-100 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Rhesus Macaque CD19 (20-292) Fc Chimera by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Rhesus Macaque CD19 Protein, C-Fc Tag
The purity of Rhesus Macaque CD19 Fc Chimera was determined by SDS-PAGE under reducing (R) condition and staining overnight with Coomassie Blue.
 
 

Background

B-lymphocyte antigen CD19 (CD19) is also known as B-lymphocyte surface antigen B4, Differentiation antigen CD19 and T-cell surface antigen Leu-12, which is a single-pass type I  membrane protein and contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. CD19 interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. CD19 is an important pan B cell marker and co-stimulatory protein in humans and mice. CD19 is a hallmark differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 defines signaling thresholds for the Ag receptor as well as other cell surface receptors that regulate B lymphocyte selection, activation, and differentiation. Defects in CD19 are a cause of hypogammaglobulinemia.

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References