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Cynomolgus CD3 epsilon & CD3 delta Heterodimer Protein, His Tag & Flag Tag  pdf  pdf  pdf


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Synonym

CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon

Source

Recombinant Cynomolgus CD3E & CD3D heterodimer protein (Cynomolgus CD3E & CD3D heterodimer) Gln 22 - Asp 117 (CD3E,Q95LI5) & Phe 22 - Ala 105 (CD3D,Q95LI8) was produced in human 293 cells (HEK293) at ACROBiosystems.

Predicted N-terminus: Gln 22 (CD3E) and Phe 22 (CD3D)

Molecular Characterization

CD3E(Gln 22 - Asp 117)Q95LI5
Poly-his
CD3D(Phe 22 - Ala 105)Q95LI8
Flag

The protein contains two subunits. Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus.

The protein has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). The protein migrates as 19-22 kDa and 25-40 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Cynomolgus CD3E & CD3D, His Tag & Flag Tag (Cat. No. CDD-C52W4) SDS-PAGE gel

Cynomolgus CD3E & CD3D, His Tag & Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity

Cynomolgus CD3E & CD3D, His Tag & Flag Tag (Cat. No. CDD-C52W4) ELISA bioactivity

Immobilized SP34-2 at 5 μg/mL (100 μL/well) can bind Cynomolgus CD3E & CD3D, His Tag, Flag Tag (Cat. No. CDD-C52W4) with a linear range of 0.6-5 ng/mL (Routinely tested).

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Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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