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Human Dkk-1 Protein, Strep Tag  pdf  pdf  pdf


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Synonym

DKK1, SK

Source

Recombinant Human DKK-1 /DKK1 Protein, C-Twin Strep Tag (rh DKK1, Twin Strep Tag) Thr 32 - His 266 (Accession # NP_036374.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh DKK1, Twin Strep Tag is fused with Twin-Strep tag at the C-terminus, and has a calculated MW of 28.8 kDa. The predicted N-terminus is Thr 32 or Ser 35. DTT-reduced Protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh DKK1, Twin Strep Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human DKK-1 /DKK1 Protein, C-Twin Strep Tag
The purity of rh DKK1, Twin Strep Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

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References