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Human EpCAM / TROP1 Protein, Fc Tag  pdf  pdf  pdf


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EPM-H5254-100ug
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EPM-H5254-1mg
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Synonym

EPCAM,TACSTD1,TROP1,CD326,DIAR5,EGP2,EGP314,
EGP40,ESA,GA733-2,HNPCC8,KS1/4,KSA,M4S1,MIC18,MK1,HNPCC-8,

Source

Human EpCAM, Fc Tag (EPM-H5254) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1).

Predicted N-terminus: Gln 24

Molecular Characterization

EpCAM(Gln 24 - Lys 265)AAH14785.1
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 54 kDa. The protein migrates as 60-65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>98% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human EpCAM, Fc Tag (Catalog # EPM-H5254) SDS-PAGE gel

Human EpCAM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

 

Background

EpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.

References

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